FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754546
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG16600.046.4323463
CCGAGTA17600.045.129332
AGTAGCT64050.040.8715325
GTAGCTG64650.040.2741246
GAGTAGC41300.040.170714
CCCGAGT21450.039.7421071
GCTGGGA113250.038.2644049
TAGCTGG70950.038.023337
TACTCGG11400.036.6918873
CTACTCG11900.034.7552642
TTGGGAG84800.034.4730266
TACGCGC550.002718187934.1807943
GGAGGCT81300.033.010179
GCTACTC29500.032.40961
AAAGTGC59450.032.0966034
AAGTGCT61500.030.7974535
GGGAGGC112950.030.1261068
TCGCGTT1102.971501E-629.9081955
GCACTTT69900.029.7139971
TTTGGGA84900.029.1180935
Produced by FastQC (version 0.11.5)