FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754546
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA17000.043.38982
CGAGTAG16500.042.9967583
CTACTCG12200.040.8209342
TACTCGG12600.040.6442033
AGTAGCT60000.040.4060975
GAGTAGC39200.039.552724
GTAGCTG61550.038.6257326
TTGGGAG91900.037.9352236
GCTGGGA113850.037.7593889
CCCGAGT21000.036.6994741
AAAGTGC58800.036.1168254
TAGCTGG68000.035.995997
GCACTTT75050.035.6910551
GGAGGCT89500.035.4337929
CCCAAAG75850.034.3852841
AAGTGCT62550.034.176895
CGGAGGT19050.034.0337227
TTTGGGA88900.033.8769875
TCGCGTT1256.2955223E-933.828365
ACTTTGG79650.033.268713
Produced by FastQC (version 0.11.5)