FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6339870
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24250.065.1600653
TACTCGG30850.064.125613
CCGAGTA26300.063.1555752
CTACTCG30850.063.1168482
GAGTAGC61450.059.3251344
GCTACTC78000.056.5570261
AGTAGCT106100.054.236645
GCTGGGA235250.054.1862379
GGAGGCT184000.053.7257849
GTAGCTG108000.053.05296
TCGGGAG41750.052.749716
CCCGAGT34650.051.5363431
CTCGGGA38800.051.468295
TTGGGAG167150.050.4320036
TAGCTGG118300.049.642897
AAAGTGC152850.048.1077464
ACTCGGG43650.046.8893974
AAGTGCT160350.046.86675
AGTGCTG157750.046.4962236
GTGCTGG157300.046.391587
Produced by FastQC (version 0.11.5)