FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624395
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG704.558986E-640.3859941
CGTACGT608.811034E-539.178842
CCGAGTA10050.038.360182
CGAGTAG9850.038.1779063
CTACTCG8200.036.694332
CCCGAGT13150.036.1887171
TTGGGAG54400.035.773366
AGTAGCT37450.035.772675
TACTCGG8200.035.5415343
GAGTAGC23100.034.390074
GTAGCTG39800.033.660476
GCACTTT46000.032.1624681
GCTACTC18950.031.8257261
GCTGGGA72100.031.8067139
TAGCTGG42900.031.0072087
GGAGGCT52850.030.6766769
CCCAAAG45350.030.3377321
GGGAGGC71650.030.0388648
TTTGGGA56300.029.4730225
TGGGAGG73000.028.9105937
Produced by FastQC (version 0.11.5)