FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15265775
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG85900.081.383033
CCGAGTA91450.077.489122
CTACTCG119700.077.394982
TACTCGG125050.076.049183
GAGTAGC210150.075.8550644
GCTACTC292800.074.092991
AGTAGCT339400.073.0166555
GCTGGGA854400.070.6429
GTAGCTG357700.069.070266
GGAGGCT712500.065.681869
TCGGGAG159350.065.333996
AAAGTGC560750.064.148434
AAGTGCT572800.064.074235
TAGCTGG398000.063.9632847
CCCGAGT111950.063.8026241
CCAAAGT599350.062.1585042
CCCAAAG654150.060.952581
CTCGGGA161500.060.7451135
TTGGGAG520650.060.368576
AGTGCTG595200.060.274176
Produced by FastQC (version 0.11.5)