FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028067
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13700.043.552732
CGAGTAG13900.042.9260753
TACTCGG12200.039.2800983
GAGTAGC32950.038.498224
CTACTCG11900.038.2963182
AGTAGCT47500.038.27745
CCCGAGT16900.037.5797121
TTGGGAG73800.036.729576
GCTACTC29750.036.5284651
GTAGCTG50850.036.400026
GCTGGGA100400.035.8419349
GGAGGCT78050.035.2110829
TAGCTGG54300.034.0873157
CGCCGTA1554.0017767E-1133.45039494
GCACTTT60900.033.1395341
AAAGTGC49050.032.47084
TTTGGGA69500.032.2447245
GGGAGGC104950.031.2889618
ACTTTGG62550.031.2462773
CTCGGGA16900.031.135545
Produced by FastQC (version 0.11.5)