FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3608078
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11000.033.79032
CGAGTAG10850.032.9533233
CTACTCG11100.029.2471622
GCTGGGA74900.028.988389
TACTCGG11500.028.6362133
GGAGGCT63400.028.1681379
AGTAGCT41650.028.1219585
GTAGCTG41200.028.0612956
TTGGGAG64250.027.795876
CCCGAGT16450.027.7717651
GCTACTC25350.027.3109631
CTCGGGA11850.026.9930385
TAGCTGG46450.026.306167
GGGAGGC77350.026.1870618
CGGAGGT11500.026.154837
CGCGAGT1458.5039756E-725.9848181
TCGCGTT1301.0816277E-525.3288765
GAGTAGC28400.025.0103514
TCGGGAG13200.024.2105586
GGAGGTT31250.024.0624458
Produced by FastQC (version 0.11.5)