FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832513
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10850.048.089943
CCGAGTA11250.047.640322
CCCGAGT14550.043.7110631
TACTCGG10200.040.0940633
GAGTAGC28800.039.335584
AGTAGCT46400.039.3074345
CTACTCG10150.037.0549552
GTAGCTG48900.036.5288286
GCTGGGA88100.036.0592589
TAGCTGG51900.035.4111257
TTGGGAG64200.033.9005666
GCTACTC22750.033.547041
TCGCGTT600.0041590431.337895
GGAGGCT61800.031.025429
TCGGGAG13500.030.6414936
AAAGTGC41650.030.585484
AAGTGCT42950.029.988065
CGGAGGT12850.029.6286757
GGGAGGC83350.029.4313988
CCCAAAG53300.029.2564331
Produced by FastQC (version 0.11.5)