FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814597
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT857.77618E-944.3376165
CCGAGTA11400.041.7418752
CGAGTAG11700.038.653313
GAGTAGC27700.037.4148644
CCCGAGT15300.034.8044931
AGTAGCT44600.034.2224735
GTAGCTG45700.033.942286
GCTGGGA75600.032.69469
TACTCGG8300.032.351773
TAGCTGG49950.031.9856747
TTGGGAG56900.031.3090726
CTACTCG8500.031.04022
CTCGCGT1551.4842954E-930.392724
GGAGGCT52750.028.7734649
GGGAGGC70250.028.4285478
GCACTTT48150.027.8931031
CTCGGGA9950.027.4603585
ACTCGCG1406.246519E-726.9192683
GCTACTC22100.026.8674551
CCCAAAG49850.026.4692211
Produced by FastQC (version 0.11.5)