FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814597
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79550.28263371274821936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT708.595998E-847.013045
CCGAGTA10950.039.0715982
CGAGTAG11200.036.5223273
CCCGAGT14000.034.621631
CGGAGGT12500.034.5632367
GCTGGGA71250.034.4654169
GAGTAGC25850.034.1925244
TTGGGAG58850.033.7664836
AGTAGCT40700.033.4982385
GTAGCTG41000.032.8844036
TAGCTGG45700.032.266367
GGAGGCT59500.031.6441979
GGGAGGC78400.029.345858
GGAGGTT34550.029.0825488
AGGCGGA16950.028.292054
TACTCGG10000.028.210353
GCTACTC23400.028.1546761
TTTGGGA58900.027.6171685
CCGGGTT7500.027.5459738
TGGGAGG78950.027.361657
Produced by FastQC (version 0.11.5)