FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511275
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98890.2816355882122591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA15300.040.556682
CGAGTAG14300.040.10723
AGTAGCT50700.038.4775665
CCCGAGT17850.038.219381
GAGTAGC33800.038.109434
GTAGCTG53400.036.7682046
GCTGGGA97250.036.4996839
TTGGGAG74850.035.3935976
TACTCGG12100.034.189753
CTACTCG11800.032.6671872
TAGCTGG59150.032.6277777
GGAGGCT72600.032.4656459
CGGAGGT15900.031.3046747
GCTACTC29750.030.8390871
GGAGGTT39550.030.3913768
AAAGTGC52150.030.2889044
AAGTGCT53850.030.0289525
GGGAGGC99500.029.8701618
GCACTTT67200.029.4058571
TTTGGGA74450.028.9811385
Produced by FastQC (version 0.11.5)