FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13944961
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG65150.077.248163
CCGAGTA67950.074.786272
TACTCGG97300.070.735673
CTACTCG97900.069.5572052
GAGTAGC165550.065.852944
GCTACTC236900.065.839021
GCTGGGA687400.064.227299
AGTAGCT276850.063.9686625
GTAGCTG287450.061.089256
CCCGAGT87150.060.8030131
TCGGGAG123300.059.4602246
GGAGGCT563200.057.9359559
AAAGTGC461450.057.508694
CTCGGGA123050.056.9280625
AAGTGCT474700.056.8920365
CCCAAAG563450.056.2821241
CCAAAGT519000.055.602472
AGTGCTG483200.055.483076
ACTCGGG124450.055.467934
TAGCTGG333300.054.949357
Produced by FastQC (version 0.11.5)