FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13944961
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG69800.077.259613
TACTCGG99750.073.832973
CCGAGTA75400.073.776272
CTACTCG99150.071.94782
GAGTAGC176200.067.849674
GCTACTC242800.067.484221
GCTGGGA689800.066.7611549
AGTAGCT281650.065.963575
GTAGCTG290450.064.175286
CCCGAGT92550.062.467871
GGAGGCT569850.060.9565549
TCGGGAG131000.060.060946
CTCGGGA125400.059.0016985
AAGTGCT476950.058.4580355
AAAGTGC467800.058.147964
TAGCTGG330850.057.83937
CCCAAAG559350.056.896241
ACTCGGG127250.056.647774
AGTGCTG492400.056.457676
TTGGGAG441950.056.2706456
Produced by FastQC (version 0.11.5)