FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705483
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA14650.044.583312
TCGCGTT1700.044.2254335
CGAGTAG14650.042.0173653
CCCGAGT18750.038.848861
TTGGGAG89800.038.8265276
CTACTCG11950.038.534822
GAGTAGC36350.038.005284
GCTGGGA104300.037.5730869
AGTAGCT56000.036.7525145
CGGAGGT15750.036.398237
TACTCGG12550.036.31813
GTAGCTG57900.036.195736
GCACTTT72600.036.1198121
CTCGCGT2100.035.8015374
GGAGGCT84400.034.5735749
TAGCTGG63750.034.127297
GCTACTC31850.033.788891
TCGGGAG18150.033.6564066
GGGAGGC118150.033.566338
TTTGGGA90600.033.1934785
Produced by FastQC (version 0.11.5)