FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092031
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9800.038.4487273
CCGAGTA10750.037.6858632
TACTCGG9300.033.9320373
AGTAGCT41900.033.7212945
CTACTCG9300.031.9114172
GTAGCTG43000.031.699666
TCGCGTT753.2372717E-431.398275
GCTGGGA71400.031.3276969
GAGTAGC26700.030.6941834
TTGGGAG58100.029.5285286
CCCGAGT14300.029.3223511
TAGCTGG47200.029.171077
CGGTCGT650.006087537428.9891262
GGAGGCT54650.027.601629
GCTACTC22450.026.0225681
GGGAGGC71450.025.978958
CGGAGGT10100.025.5898697
GCACTTT50600.025.512021
TCGGGAG12350.025.4995736
CTCGCGT1301.0708598E-525.3613744
Produced by FastQC (version 0.11.5)