FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092031
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90180.29165296208220426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1001.4100551E-632.906355
CGGAGGT11850.031.704077
TTGGGAG62650.030.964516
CCGAGTA10150.030.5679742
GGAGGCT58650.030.4225339
AGTAGCT39900.030.2790225
CTACTCG7800.030.134512
TACTCGG8650.029.891623
GCTGGGA72000.029.6728129
GTAGCTG40000.029.2398136
GGGAGGC77700.028.4029668
GAGTAGC25750.027.7499494
CGAGTAG10700.027.679593
TGGGAGG81050.026.8848937
GGCGGAG18400.026.8258295
GCTACTC22500.026.7621171
AGGCGGA18750.026.5766624
GGAGGTT34350.026.5192898
CTCGGGA9400.026.0050185
TAGCTGG45700.025.8957027
Produced by FastQC (version 0.11.5)