FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3202572
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90650.28305374555201257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1201.8189894E-1243.097555
CGCGTTG1400.040.264196
ACTCGCG1601.8189894E-1235.263853
CTCGCGT1651.8189894E-1234.194174
CCGAGTA10800.033.9561772
CGGAGGT11950.033.403667
CGAGTAG10650.032.670113
AGTAGCT39900.031.9328385
TAGCGCG600.004155362531.3436725
GAGTAGC25750.031.222934
TACTCGG10250.031.1927383
CTACTCG10050.030.876342
GTAGCTG41200.030.6704986
AGGCGGA17550.030.2730464
GCTGGGA74350.030.060829
TCGGGAG12750.029.4744436
TTGGGAG57450.029.1906646
GGAGGCT58400.029.1051989
CGCCGTA1303.3173455E-728.94035594
CCCGAGT16000.028.8201051
Produced by FastQC (version 0.11.5)