FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686412
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75070.2794433616288194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9050.036.884962
CGAGTAG9700.033.9292263
CGGAGGT10250.032.9870837
AGTAGCT35050.032.5924575
CCCGAGT11900.032.420821
TACTCGG8450.032.271493
GCTGGGA63950.031.8624259
GAGTAGC22650.031.5505374
CTACTCG8300.031.154742
TTGGGAG53150.031.0196366
GCTACTC21950.030.8663311
GTAGCTG35950.030.5738286
GGAGGCT53700.029.3761339
CCCAAAG43600.027.40971
TAGCTGG41150.027.046677
GGGAGGC69350.026.7416618
CCCGAAT4300.026.2603111
GGAGGTT30900.025.982178
TGGGAGG67450.025.899797
GGCGGAG17350.025.7408565
Produced by FastQC (version 0.11.5)