FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15197472
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG71400.066.009563
CTACTCG72950.063.791322
CGAGTAG50800.059.7186663
GCTACTC188800.058.168221
CCGAGTA54400.058.1497882
GGAGGCT440800.054.902629
GCTGGGA521550.054.6725589
GAGTAGC130750.052.982174
TCGGGAG93650.052.9296576
CTCGGGA92800.052.1739735
CGGAGGT103250.048.2700587
ACTCGGG98800.048.1830374
CCCGAGT75900.048.171251
AGTAGCT232100.048.169015
GTGCTGG390100.047.9964687
AAAGTGC396700.047.642384
CCCAAAG473700.047.0119631
AGTGCTG405650.046.6079256
AAGTGCT415850.046.4254725
GTAGCTG238300.045.921196
Produced by FastQC (version 0.11.5)