FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10325552
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG65000.082.90693
CCGAGTA68300.078.65872
GAGTAGC159500.077.202994
AGTAGCT253450.074.572375
TACTCGG79600.073.505583
GTAGCTG262650.071.786836
CTACTCG78800.071.648382
GCTACTC187300.069.2287141
GCTGGGA586550.068.52999
CCCGAGT80050.067.02361
TAGCTGG301400.064.522477
GGAGGCT455450.062.433729
TCGGGAG104400.061.156446
TTGGGAG350750.059.118276
AAAGTGC353900.058.92314
CCAAAGT378600.058.7385942
AAGTGCT362400.058.6365855
CTCGGGA103250.057.929415
ACTCGGG101800.057.411944
CCCAAAG420600.057.4104541
Produced by FastQC (version 0.11.5)