FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15702595
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG89900.069.140443
CTACTCG88600.067.082782
CGAGTAG60300.065.306773
CCGAGTA66050.062.449442
GCTACTC224900.061.5427551
TCGGGAG114900.058.8138856
GCTGGGA633650.058.2381369
GAGTAGC160600.056.901324
GGAGGCT519950.056.696889
CTCGGGA113050.056.4992645
CCCGAGT87200.053.1190571
ACTCGGG118950.052.8893934
AGTAGCT273050.052.7894975
GTAGCTG279550.051.390266
AAAGTGC456900.050.7226754
GTGCTGG457750.050.4629827
AAGTGCT474750.050.0005535
CCCAAAG565700.049.820781
AGTGCTG472450.049.4017266
CGGAGGT116800.048.164737
Produced by FastQC (version 0.11.5)