FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15702595
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG92600.067.49033
CGAGTAG61750.067.1017763
CTACTCG90850.065.920932
CCGAGTA63900.065.9176252
GCTACTC218200.062.923391
GCTGGGA626950.060.7064449
GAGTAGC158150.059.6007274
CCCGAGT80150.057.871261
TCGGGAG113600.055.3760876
GGAGGCT524900.054.995919
AGTAGCT274400.054.4014135
GTAGCTG276750.053.6662836
AAAGTGC448950.053.5579644
CTCGGGA114250.053.26025
CCCAAAG553000.052.0879251
AAGTGCT469000.051.882845
GTGCTGG457700.051.649447
AGTGCTG466400.051.2302176
ACTCGGG118600.051.07524
TAGCTGG310600.050.1366927
Produced by FastQC (version 0.11.5)