FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012688
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8800.034.1668432
TACTCGG9350.034.1668433
TCGCGTT1107.73307E-834.1668435
CGTCGCG702.1916855E-433.5561649
CGAGTAG10800.033.4945763
GAGTAGC22800.032.7619554
TTGGGAG53450.032.6076356
CCGAGTA12000.032.494092
GCTGGGA69300.031.793629
CGGAGGT11350.031.4565337
GCTACTC22050.031.1525671
GGAGGCT54550.031.0894329
AGTAGCT37150.030.6030085
GTAGCTG37300.030.2270346
CCCGAGT14800.029.246621
CGTACCG650.006168920528.9104063
TAGCTGG43800.028.5299247
GGGAGGC73800.028.390418
CTCGGGA12000.027.013165
TGGGAGG75850.026.3224627
Produced by FastQC (version 0.11.5)