FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7931232
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32500.058.113893
TACTCGG35950.056.630683
CCGAGTA35350.054.4927942
CTACTCG36950.053.793452
GAGTAGC84500.053.556514
GCTACTC91550.051.6561161
AGTAGCT140100.050.8167275
GCTGGGA285700.050.7191059
GTAGCTG143450.049.2880176
TAGCTGG155100.047.720847
CCCGAGT45350.047.4001961
GGAGGCT224850.046.9359979
TTGGGAG203800.045.7353486
CTCGGGA47350.042.0897145
AAAGTGC184450.041.822944
CGGAGGT50750.041.8073547
CCCAAAG229100.041.525581
AAGTGCT188950.041.3461575
TCGGGAG48000.041.136496
CCAAAGT207350.040.8886572
Produced by FastQC (version 0.11.5)