FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7931232
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA35250.060.7039452
CGAGTAG33850.059.2304653
CTACTCG39450.057.1941222
TACTCGG40850.056.8857153
GAGTAGC83200.055.4916654
AGTAGCT140050.054.1555185
GCTGGGA286450.054.0901229
GTAGCTG140400.053.497146
GCTACTC97200.053.2483561
CCCGAGT44850.052.9149671
TAGCTGG153750.050.8456577
GGAGGCT233850.050.3938529
TTGGGAG209800.047.899956
CGGAGGT52000.047.754087
TCGGGAG51850.045.527476
CTCGGGA49900.045.09435
AAAGTGC186550.043.5159844
AAGTGCT187900.043.4970175
ACTCGGG52700.043.1637734
GTGCTGG187250.042.9933937
Produced by FastQC (version 0.11.5)