FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258315
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13100.038.8381232
CGAGTAG12950.038.191953
GTAGCTG49650.036.5409166
AGTAGCT48850.035.6770945
CCCGAGT17000.034.9248281
ACGATCG550.002719980234.1760678
GAGTAGC30500.034.1306654
GCTGGGA86950.033.5078749
TAGCTGG57050.031.9595647
CTACTCG9000.031.929572
CGGAGGT10950.031.7572127
TACTCGG9050.031.2287753
TTGGGAG63350.030.1224866
GGAGGCT58150.028.2033359
GCTACTC24150.027.3164041
GGAGGTT34050.027.049788
GGGAGGC76300.026.8526258
AGCTGGG73550.025.620438
GCGGAGG14700.025.579036
CCCAAAG58950.025.578721
Produced by FastQC (version 0.11.5)