FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258315
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91330.28029825231753225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG555.244935E-542.7827381
CCGAGTA11100.040.2475662
TCGCGTT1450.038.9139485
GAGTAGC31000.038.681574
AGTAGCT47100.038.535275
CGAGTAG10700.037.7955133
GTAGCTG48050.036.367976
TTGGGAG71350.036.2108086
GCTGGGA84300.034.70219
CGTACCG550.002710756134.1997453
TACTCGG9600.033.309133
ACTCGCG1703.6379788E-1233.193873
TAGCTGG53550.032.532427
CTACTCG9600.031.3507392
CCCGAGT16450.031.1832851
GGAGGCT69400.031.1238739
GGGAGGC92500.030.6612958
TTTGGGA73150.030.4045725
GTACGCT953.7409485E-529.69978194
GCACTTT63050.029.632391
Produced by FastQC (version 0.11.5)