FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176826
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12300.040.226982
CGAGTAG12100.040.1098253
CCCGAGT14850.036.5012251
AGTAGCT42700.035.4222455
GAGTAGC29150.034.7535254
CGGAGGT10250.033.9252177
GCTGGGA78150.033.7325139
GTAGCTG43200.033.7304886
TTGGGAG61050.031.798686
TAGCTGG47600.031.3931587
TACTCGG8750.028.5408423
GCGGAGG14350.028.1702866
GGGAGGC79600.027.450788
GGCGGAG16650.027.1678925
GGAGGCT59800.027.0316229
TCGCGTT1406.234677E-726.925325
CTACTCG8900.026.4736142
CGCGTTG1458.650386E-725.933836
GCACTTT57800.025.931961
TTTGGGA64500.025.8608325
Produced by FastQC (version 0.11.5)