FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176826
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91570.2882436746614388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12850.040.6156433
CCGAGTA13000.040.5093272
CGGAGGT12700.035.500317
TACTCGG10650.034.8779533
CCCGAGT16600.033.7347261
AGTAGCT47100.033.639925
GCTGGGA80450.033.5628139
CTACTCG10450.033.2962882
TTGGGAG71150.032.94796
GTAGCTG49050.032.7558756
GGAGGCT68050.031.949899
GAGTAGC32350.031.53984
TAGCTGG52350.030.8607437
GCTACTC26100.030.6511461
GGGAGGC90400.029.453118
GGAGGTT35900.029.4309718
GCACTTT59100.029.3817211
TCGCGTT1154.1883104E-628.6184565
CCCAAAG54750.028.3640421
CACTTTG62200.027.743172
Produced by FastQC (version 0.11.5)