FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3007568
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10750.035.8698922
CGAGTAG10650.035.3159683
ATGCGCG902.589886E-531.3429244
CCCGAGT15250.030.9118771
CTACTCG8100.030.1886022
TACTCGG8500.029.8679623
TTGGGAG56050.028.7706246
AGTAGCT38850.028.5595765
GGAGGCT51850.028.279429
GCTGGGA69750.028.0293989
GAGTAGC25550.027.9694214
CTCGCGT1205.8334044E-627.425064
GTAGCTG38700.027.2124636
GGGAGGC68450.026.5019918
TAGCTGG43600.026.3073027
CTCGGGA10300.025.561225
GCTACTC18650.025.2764681
TGGGAGG70750.024.3179877
TCGGGAG11250.024.2385276
CGGAGGT9950.024.0946227
Produced by FastQC (version 0.11.5)