FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813584
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109460.2870265870635077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11150.037.5301783
CTACTCG11350.037.2836042
CGGAGGT13300.033.542757
TTGGGAG72850.029.9209636
AGTAGCT47100.029.7446735
GCTGGGA87100.029.4875479
CTCGGGA13450.029.3610025
GTAGCTG47600.029.3114076
GGAGGCT69600.029.1436529
GCTACTC29250.028.7936481
TAGCTGG54900.027.0297167
GGGAGGC94300.026.6891068
GAGTAGC31050.026.4983584
AGGCGGA21700.026.4327664
GCGGAGG17500.025.770356
TCGGGAG16300.025.650136
CGAGTAG14050.025.0986523
CCCAAAG67550.025.075331
GGAGGTT39700.025.0741528
GGCGGAG21350.024.8825245
Produced by FastQC (version 0.11.5)