FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10608135
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG48900.070.867343
CCGAGTA51700.069.059872
CTACTCG64050.064.131032
TACTCGG64950.064.046273
GCTGGGA462750.062.115359
GAGTAGC121800.061.517434
GCTACTC158000.060.8902631
CCCGAGT61750.060.3234521
AGTAGCT202600.059.7420275
GTAGCTG207800.058.311346
GGAGGCT380300.055.787219
TCGGGAG79850.054.7638176
TAGCTGG234500.054.0715377
AAAGTGC318250.053.4469574
CCCAAAG382550.052.9889871
AAGTGCT325300.052.8320165
CTCGGGA80600.052.657155
AGTGCTG323150.052.406416
CCAAAGT355200.051.9373132
GTGCTGG326200.051.6743747
Produced by FastQC (version 0.11.5)