FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10608135
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG49800.075.101293
CCGAGTA52950.073.851842
TACTCGG66300.072.119053
CTACTCG66350.070.388472
GAGTAGC126800.066.0164644
GCTGGGA474550.065.620049
CCCGAGT64000.064.1888351
GCTACTC163250.064.054791
AGTAGCT210000.063.065855
GTAGCTG212600.062.15586
GGAGGCT394200.059.6285749
TCGGGAG85200.058.25776
AAAGTGC317300.057.841394
AAGTGCT325700.057.3138435
TAGCTGG240950.057.03617
AGTGCTG326050.056.4145746
CTCGGGA83550.056.1305125
GTGCTGG325350.056.0854847
ACTCGGG85550.055.8146484
CCCAAAG383900.055.672121
Produced by FastQC (version 0.11.5)