FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682139
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG38950.060.3781173
CCGAGTA41350.058.6052442
TACTCGG43100.058.1819763
CTACTCG44700.055.0851942
GAGTAGC97650.054.2738844
GCTACTC114600.054.1394581
GCTGGGA342900.053.980449
CCCGAGT52400.052.4999961
GGAGGCT272300.051.625619
AGTAGCT165050.051.1564945
GTAGCTG166200.049.9490856
TAGCTGG185150.047.2169887
CGGAGGT59950.045.5771687
TTGGGAG234900.045.5644046
TCGGGAG57850.044.8791546
CCCAAAG293250.044.2662471
GTGCTGG242400.043.990547
AGTGCTG242850.043.7787976
AAAGTGC241150.043.7123574
AAGTGCT248450.043.316815
Produced by FastQC (version 0.11.5)