FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682139
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG37700.066.322753
TACTCGG46750.063.06923
CCGAGTA42300.062.0277982
CTACTCG47200.060.954682
GAGTAGC95150.059.995814
GCTGGGA348450.058.3679289
GCTACTC116650.056.3058321
CCCGAGT50300.055.7959561
AGTAGCT166850.055.737945
GTAGCTG167350.055.3213926
TAGCTGG182150.052.0331737
GGAGGCT279100.051.4493189
TCGGGAG62800.049.1058546
GTGCTGG244450.048.1199077
AAAGTGC246150.048.0980724
CCCAAAG303900.047.6717531
TTGGGAG240050.047.3839956
CTCGGGA63000.047.2127235
AAGTGCT255650.046.94515
AGTGCTG250450.046.8680276
Produced by FastQC (version 0.11.5)