FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9107035
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG48350.080.969633
CCGAGTA51250.076.893822
TACTCGG63850.075.931583
CTACTCG63950.074.6979452
GAGTAGC120100.072.425454
AGTAGCT197200.069.844745
GCTACTC152200.068.404041
GTAGCTG203750.067.090816
GCTGGGA452050.066.930829
CCCGAGT64500.064.524731
GGAGGCT381050.061.677619
TCGGGAG82600.061.1281856
TAGCTGG233300.060.7871637
CTCGGGA80100.060.365395
TTGGGAG308300.059.917746
AAAGTGC288150.059.5411224
AAGTGCT293400.058.5861665
ACTCGGG81900.058.1681374
CCCAAAG353900.056.9679641
CCAAAGT325650.056.7052272
Produced by FastQC (version 0.11.5)