FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034848
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG30750.057.2818373
CCGAGTA27800.052.712392
CGAGTAG26300.052.1692353
CTACTCG32550.051.6686442
GCTACTC83150.050.9297071
GCTGGGA237100.050.6478659
GAGTAGC68450.047.4929924
CGGAGGT44300.047.434297
GGAGGCT201000.047.2296879
TTGGGAG183450.046.593116
AGTAGCT117150.046.425615
CCCGAGT38700.046.074311
GTAGCTG117150.046.0563166
CTCGGGA39800.043.169695
TCGCGTT2650.041.9801335
TAGCTGG132900.041.7607777
CGTCGCA2550.041.202735
GGGAGGC245000.041.0429578
CCCAAAG206000.041.024451
TCGGGAG42700.040.6720126
Produced by FastQC (version 0.11.5)