FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034848
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG33850.061.1572653
CTACTCG34850.060.8205762
GCTACTC88800.054.843331
CGAGTAG29050.054.6699753
CCGAGTA30650.053.0254632
GCTGGGA240800.051.9670149
GAGTAGC68950.049.742324
AGTAGCT111350.049.3380055
GGAGGCT205550.049.2141959
GTAGCTG110850.048.8918656
TTGGGAG187050.047.905246
CTCGGGA43100.047.0290955
CCCGAGT42100.046.0954551
TCGGGAG47650.046.0403026
CGGAGGT47300.044.419847
TAGCTGG128600.044.1124237
ACTCGGG47200.043.4673734
GTGCTGG168300.042.5557757
CCCAAAG214300.042.1922571
AAAGTGC172700.041.9375084
Produced by FastQC (version 0.11.5)