FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327661
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT851.7311178E-533.245125
CTCGCGT1351.3023964E-831.3981674
CCGAGTA8950.028.9481122
CGAGTAG8550.028.6439443
TACTCGG8900.028.0466793
GCTGGGA68900.027.4179849
CGGAGGT10900.027.1608127
CGCGAGT1755.4187694E-926.9228521
GAGTAGC23350.026.4228694
AGTAGCT38700.026.2868395
CTACTCG9050.024.984652
GTAGCTG39800.024.9198576
TTGGGAG55300.024.905126
GGGAGGC66300.024.5248
GGAGGCT53800.024.3697269
CCCGAGT14350.024.296231
TCGGGAG10950.024.0392066
GGAGGTT29250.023.4560958
CCCAAAG53550.023.4035241
TAGCTGG46050.022.756427
Produced by FastQC (version 0.11.5)