FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3055849
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86670.28362003489046744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9950.033.0745773
CTACTCG10300.032.4049872
GTAGCTG39400.028.8580386
AGTAGCT41400.028.6148155
GAGTAGC27000.027.6850664
CGAGTAG11900.027.6547933
GCTGGGA69050.027.000669
CCGAGTA11700.026.5184942
CTCGGGA12150.025.923245
CGGAGGT12250.025.3049477
TCGGGAG13000.025.298946
CCCGAGT14150.024.601781
TAGCTGG47650.024.543367
GGAGGCT54000.023.6549879
TAGTCCG1401.9378496E-523.4918736
TTGGGAG50300.022.8847276
ACTCGGG14800.022.8708864
CGCCGTA1502.8242826E-521.94561694
GGAGGTT32000.021.867098
GGGAGGC66300.021.1085038
Produced by FastQC (version 0.11.5)