FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9041780
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32050.056.0183943
CCGAGTA35550.055.3131262
TACTCGG35800.055.2535863
CTACTCG35250.054.1747252
CGGAGGT50150.050.497957
GCTGGGA293650.050.0719229
GAGTAGC87600.049.2606664
GCTACTC97150.049.163171
AGTAGCT143550.048.4923135
CCCGAGT49000.048.2860871
GGAGGCT230250.047.4157189
GTAGCTG145250.047.360946
TAGCTGG161900.044.825417
CTCGGGA44300.044.083075
TTGGGAG212050.043.5521436
TCGGGAG48950.041.1823466
GTGCTGG194850.041.1572727
AGGCGGA75850.041.0284584
GGCGGAG75800.040.80625
GGGAGGC282400.040.153788
Produced by FastQC (version 0.11.5)