FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9041780
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG33700.063.3631673
CCGAGTA36800.061.620012
TACTCGG39050.061.1343423
CTACTCG39800.056.1839642
CCCGAGT48050.054.4112931
GCTGGGA299950.053.486579
GAGTAGC88500.053.3096244
CGGAGGT54600.051.7983557
AGTAGCT146000.051.599495
GTAGCTG145700.050.668166
GGAGGCT243400.050.0495959
CTCGGGA46900.049.155865
TAGCTGG165300.046.565117
TCGGGAG51850.045.920876
TTGGGAG222600.045.4734426
GTGCTGG197850.044.507527
AAGTGCT205700.044.4018255
AAAGTGC203250.044.193264
ACTCGGG54950.043.7887734
AGTGCTG202450.043.7456256
Produced by FastQC (version 0.11.5)