FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136822
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11600.036.5440443
CCGAGTA12650.034.6310922
AGTAGCT43550.032.1213265
CTACTCG8700.031.9452652
TCGCGTT753.2360497E-431.4003095
GTAGCTG46050.031.232166
CGGAGGT10850.031.1833677
GCTGGGA79250.030.4778359
TAGCTGG48050.030.2193667
CCGCGAT953.697192E-529.7509862
TACTCGG8950.029.4711513
GAGTAGC30300.028.9131534
CCCGAGT17150.028.2972341
TTGGGAG61150.028.208496
GGAGGCT58900.027.3652279
GGGAGGC75650.026.5861288
GCTACTC20450.026.0349081
CTCGGGA11400.025.6160415
GGCGGAG15750.024.8211965
CGTCGAG950.001286430824.7980371
Produced by FastQC (version 0.11.5)