FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136822
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89570.2855437764718559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9950.034.9659922
TTGGGAG59300.034.145416
CGGAGGT13100.034.0593767
TACTCGG10650.033.9904943
CGAGTAG11550.032.5629963
TCGCGTT1457.4396667E-1032.4195145
CCGAGTA12800.031.9555682
AGTAGCT42450.031.2281255
GCTGGGA78100.031.2089989
GCTACTC26050.031.0630321
GGAGGCT61900.030.8792889
GTAGCTG43300.030.704946
GGCGGAG19550.030.296915
CCCGAGT15900.029.8845961
GAGTAGC28500.029.8567894
ACTCGCG1751.70985E-1029.5509173
CTCGCGT1602.1318556E-929.3825444
TAGCTGG48350.028.6556267
GGGAGGC81250.028.4962068
CTCGGGA13900.027.7315125
Produced by FastQC (version 0.11.5)