FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073436
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT751.478893E-743.8786435
TACTCGG8550.035.7407533
CTACTCG8100.035.4047282
CGGAGGT11900.034.7476967
TTGGGAG54500.033.8822486
GTAGCTG36050.031.0204326
CCGAGTA9900.030.3921342
GCTGGGA66050.030.1665559
AGTAGCT35500.030.0617285
GGAGGCT55100.029.5944679
CGAGTAG9650.029.230783
TAGCTGG42550.029.0433337
GCTACTC23400.028.95691
AGGCGGA17050.028.676454
GGGAGGC73600.028.2190138
GCACTTT50800.028.0662461
TGGGAGG72900.027.587137
GGAGGTT34800.027.545358
GGCGGAG17550.027.0558195
CTTTGGG58750.026.7272974
Produced by FastQC (version 0.11.5)