FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18939897
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG173550.084.29312
TACTCGG177500.083.9008563
CGAGTAG109000.082.078253
GCTACTC405700.080.334531
CCGAGTA117250.077.366892
GAGTAGC267150.076.471694
AGTAGCT427100.074.1993265
GCTGGGA1155700.071.745149
GTAGCTG453650.070.008816
TCGGGAG230150.068.861896
GGAGGCT982500.067.711689
AAGTGCT766450.067.3959665
AAAGTGC758550.066.873684
CCAAAGT805500.066.5891952
CTCGGGA229900.066.465765
ACTCGGG226050.066.2488864
CCCAAAG892150.065.060851
CTACTTG269600.064.3382642
TAGCTGG514350.064.158537
CAAAGTG818800.063.3239823
Produced by FastQC (version 0.11.5)