FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353436
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT54550.041.042945
GAGTAGC35750.038.9441224
TTGGGAG76850.038.4088336
TACTCGG10050.038.3835683
GTAGCTG57400.038.1583256
CCGAGTA15350.038.0063552
CGAGTAG14850.037.3811873
GCTGGGA100050.037.343379
TAGCTGG62550.035.314537
CTACTCG10650.034.457832
CCCGAGT19750.034.3409081
GCACTTT64450.033.9087261
GCTACTC25950.033.759191
GGAGGCT76150.032.4005859
GGGAGGC103450.032.2105878
CCCAAAG65100.032.195521
AAGTGCT52750.032.1000945
AAAGTGC53650.031.2985884
TTTGGGA78250.031.2568555
ACTTTGG71450.031.0110323
Produced by FastQC (version 0.11.5)