FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326209
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG46050.076.118523
CCGAGTA50050.072.0122452
TACTCGG59800.067.8715743
GAGTAGC122950.065.0961154
CTACTCG61500.063.4263462
GCTGGGA458400.062.3100179
GTAGCTG205200.062.1457376
AGTAGCT206400.061.531925
GCTACTC150800.060.5872421
CCCGAGT64400.060.2315751
TAGCTGG235050.056.152237
TCGGGAG80100.054.6573076
GGAGGCT366650.053.861999
AAAGTGC305000.052.8279724
CTCGGGA79550.052.250725
ACTCGGG79750.052.1201934
AAGTGCT316050.051.7099465
CCAAAGT340600.051.168672
CCCAAAG373200.051.1021541
GTGCTGG317300.050.1647957
Produced by FastQC (version 0.11.5)