FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326209
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG46450.070.187193
TACTCGG65350.068.331743
CCGAGTA48700.068.161962
CTACTCG65650.066.31432
GCTGGGA458450.062.7648059
GAGTAGC117900.062.5107424
GCTACTC151850.062.12821
AGTAGCT197300.061.2218485
GTAGCTG203350.059.6485066
CCCGAGT62900.059.5966951
GGAGGCT381350.056.737559
AAAGTGC304250.055.4176064
TAGCTGG228150.054.9798247
AAGTGCT312000.054.8849075
TCGGGAG86600.054.595986
CTCGGGA82700.054.2345165
TTGGGAG308900.053.933276
CCCAAAG366450.053.9165761
AGTGCTG312850.053.7574776
GTGCTGG315550.052.9187977
Produced by FastQC (version 0.11.5)