FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12088286
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG72100.082.892333
CCGAGTA76900.080.0803452
CTACTCG101200.074.868692
TACTCGG104450.073.889253
GAGTAGC181100.073.597054
GCTACTC230900.071.987031
AGTAGCT291600.071.408235
GCTGGGA714400.069.696649
GTAGCTG299250.068.9984056
CCCGAGT94550.067.543871
GGAGGCT580200.063.21389
TAGCTGG340000.062.8581057
AAAGTGC460400.062.1184274
AAGTGCT471200.061.6215825
CCAAAGT502250.061.5736242
CCCAAAG544800.061.093961
TCGGGAG134250.060.7095646
CTCGGGA130500.059.8480455
TTGGGAG429600.058.80776
AGTGCTG488750.058.701846
Produced by FastQC (version 0.11.5)